Morphological, Molecular and Genomic Characterization of Two Inter-Subspecific Hybrids between Olive Cultivars and Olive Subspecies

Two inter-subspecific F1 hybrids have been obtained by crossing olive cultivars (‘Frantoio’ and ‘Coratina’) with pollen donors from subspecies (Olea europaea subsp. cuspidata) to enrich the germplasm of cultivated in southern China. This study aimed investigate characterization morphological traits molecular markers two their parents crosses. The showed a significant difference between genotypes according main discriminative parameters on qualitative quantitative leaf, fruit, endocarp. A set six co-dominant polymorphic simple sequence repeats (SSRs) were used for identification, SSR analysis confirmed that progenies offspring cited based presence parental specific alleles. Three single-copy nuclear loci (SCNL) primer pairs amplification genes after then PCR products sequenced. Sequence alignment effective data total 15 different base sites progenies, which as true inter-specific cuspidata.